| Sun, March 28 | |
| 8:00 – 10:30 pm |
Reception at the Holiday Inn Select Bethesda Potomac Room, 8120 Wisconsin Avenue Bethesda, MD 20814 301-652-2000 |
| 9:00 pm | Planning Committee Meeting |
| Mon, March 29 | |
| 7:45 am | Refreshments |
| 8:15 am | Opening Remarks – John Norvell and Jeremy Berg |
| Session I | Modification of Proteins to Optimize Expression/Solubility Chair: Stephen Anderson |
| 8:30 am | "Heterologous protein production in Escherichia coli: strategies and challenges" [PPT] [HTML] François Baneyx, University of Washington |
| 9:10 am | "Tags and Beyond" [PPT] [HTML] Barbara Morris, EMD Biosciences, Inc. |
| 9:50 am | "Protein production for structure-based drug design" [PPT] [HTML] Stephen Chambers, Vertex Pharmaceuticals |
| 10:30 am | Coffee break |
| 11:00 am | "Multiple species and computational domain prediction: A two-pronged
attack on the expression of difficult proteins from pathogenic protozoa"[PPT] [HTML] Christopher Mehlin, SGPP |
| 11:15 am | "Systematic Identification of Protein Domains for Structure Determination" [PPT] [HTML] Ming Luo, SECSG |
| 11:30 am | "Progress on directed evolution approaches and new protein solubility reporters" [PPT] [HTML] Stephanie Cabantous, TBSGC |
| 11:45 am | "A
facile method that allows high-throughput co-expression from plasmids
with identical replication origins and antibiotic resistance markers" [PPT] [HTML] Andrei Alexandrov, SGPP |
| 12:00 pm | Lunch |
| Session II | Alternative and Novel Prokaryotic Expression Systems Chair: Michael Adams |
| 1:15 pm | "Designing a Chaperonin/Osmolyte Folding Array System" [PPT] [HTML] Mark Fisher, University of Kansas Medical Center |
| 1:55 pm | "Genetic tools for metabolic enzyme production in Escherichia coli" [PPT] [HTML] Jay Keasling, University of California, Berkeley |
| 2:35 pm | "On-column chemical refolding" [PPT] [HTML] Rosalind Kim, BSGC |
| 2:50 pm | "One-step affinity purification and processing of fusion proteins" [PPT] [HTML] Philip Bryan, University of Maryland Biotechnology Institute |
| 3:05 pm | Coffee break |
| 3:25 pm | "Expression of periplasmic proteins and soluble domains of membrane proteins" [PPT] [HTML] Frank Collart, MCSG |
| 3:40 pm | "Conversion of E. coli to a protein-producing machinery by cold-shock expression vectors" [PPT] [HTML] Li-Chung Ma, NESG |
| 3:55 pm | "Rapid isolation of single-chain antibodies for structural genomics" [PPT] [HTML] Mark Sullivan, SGPP |
| 4:10 – 6:00 pm | Poster Session – Presenters have to be by their posters |
| 7:00 pm | Dinner at Foong Lin Restaurant 7710 Norfolk Avenue Bethesda, MD 20814 301-656-3427 |
| Tues, March 30 |
|
| 7:45 am | Refreshments |
| Session III | Eukaryotic Expression Systems Chair: Scott Lesley |
| 8:15 am | "Producing recombinant glycoproteins in the baculovirus-insect cell system" [PPT] [HTML] Donald Jarvis, University of Wyoming |
| 8:55 am | "Bench to clinic of biotherapeutic molecules: Issues to consider" [PPT] [HTML] Michael Meagher, University of Nebraska, Lincoln |
| 9:35 am | "Transient transfection into eukaryotic cells: an alternative to
bacterial and insect cell systems for the rapid generation of
recombinant proteins?" [PPT] [HTML] Sabine Geisse, Novartis Institutes for Biomedical Research |
| 10:15 am | "High-throughput protein production for structural investigations from a cell-free wheat germ expression system" [PPT] [HTML] John Markley, CESG |
| 10:30 am | Coffee Break |
| Mini-Session | Automation Systems |
| 11:00 am | "The development and results of routine semi-automated cloning" [PPT] [HTML] Heath Klock, JCSG |
| 11:15 am | "High-throughput protein production platform for the Northeast Structural Genomics Consortium" [PPT] [HTML] Thomas Acton, NESG |
| 11:30 am | "NYSGXRC high-throughput protein production and crystallization system" [PPT] [HTML] Jeffrey Bonanno, NYSGXRC |
| 11:45 am | Lunch |
| Session IV | Methods to Minimize Sample Heterogeneity and Improve Crystal Diffraction Chair: Jeffrey Bonanno |
| 1:00 pm | "A microfluidic system for protein crystallization using nanoliter volumes of solutions" [PPT] [HTML] Rustem Ismagilov, University of Chicago |
| 1:40 pm | "ASA-based thermodynamic analysis of solute effects on protein processes" [PPT] [HTML] Thomas Record, University of Wisconsin, Madison |
| 2:20 pm | "Solubility Optimization Screen
(SOS): An efficient screen to optimize buffer conditions for stability
and crystallization of proteins" [PPT] [HTML] Jarmila Jancarik, BSGC |
| 2:35 pm | "Enhancement of protein crystallization and crystal quality by rational surface mutagenesis: Application selected SG targets" [PPT] [HTML] Jakub Bielnicki, University of Virginia |
| 2:50 pm | Coffee Break |
| 3:20 pm | "Shotgun crystallization of the T. maritima proteome: Protein properties and crystallization conditions which correlate with crystallization success" [PPT] [HTML] Rebecca Page, JCSG |
| 3:35 pm | "High-throughput, capillary-based protein crystallography" [PDF] Deirdre Meldrum, University of Washington |
| 3:50 pm | "Growing larger protein crystals: Some insights from fundamental studies" [PPT] [HTML] Robert Thorne, Cornell University |
| 4:05 pm | "Crystallization strategy in the Midwest Center for Structural Genomics" [PPT] [HTML] Irina Dementieva, MCSG |
| 4:20 pm | Break |
| 4:35 pm | "Crystal salvaging efforts at SECSG" [PPT] [HTML] Zhi-Jie (James) Liu, SECSG |
| 4:50 pm | "Use of high throughput screening to determine lead crystallization conditions and as a tool for basic research" [PPT] [HTML] Joseph Luft, SGPP |
| 5:05 pm | "Protein crystal growth for eukaryotic pathogenic protozoa in SGPP" [PPT] [HTML] Lori Anderson, SGPP |
| 5:20 pm | "Classification and data mining of high-throughput protein crystallization screens" [PDF] Igor Jurisica, NESG |
| 5:35 – 6:00 pm | Discussion |
| Wed, March 31 | |
| 7:45 am | Refreshments |
| Session V | Membrane-Associated Proteins Chair: Rosalind Kim |
| 8:15 am | "Large-scale expression and automated purification of G-protein-coupled receptors for structure determination" [PPT] [HTML] Reinhard Grisshammer, National Institute of Diabetes and Digestive and Kidney Diseases/NIH |
| 8:55 am | "Structures of integral membrane proteins involved in transport across membranes" [PPT] [HTML] William Cramer, Purdue University |
| 9:35 am | "Genomics approach to expression of membrane proteins from Mycobacterium tuberculosis" [PPT] [HTML] Robert Nakamoto, University of Virginia |
| 9:50 am | "Expression and purification of membrane proteins: Screening of Thermotoga marititma alpha-helical membrane proteins for NMR structural studies" [PPT] [HTML] Linda Columbus, JCSG |
| 10:05 am | Coffee Break |
| 10:30 am | "Expression and purification of membrane proteins from Leishmania major for structural genomics" [PPT] [HTML] Mark Dumont, SGPP |
| 10:45 am |
"Development of methods for heterologous membrane protein production and purification" [PPT] [HTML] Han-Seung Lee, SECSG |
| 11:00 am | Summary of Meeting [PPT] [HTML] – Andrzej Joachimiak |
| 12:15 pm | End |