Sinc Shaped Band Selective Decoupling

  1. Calibrate the decoupler for the probe to be used. Use CW off-resonance decoupling and measure the residual coupling constant. Use 'h2cal' macro to get gammaH2 at a series of dpwr2 values. (assuming channel 3 is set to 13C)
  2. Example:

    dpwr2
    gammaH2
    39
    1695
    40
    1850
    41
    2043
    42
    2380
    43
    2624
    44
    2962

     
  3. Choose the bandwidth of decoupling desired and divide by 1.3 to get the gammaH2 to use.
  4. Example: The C' chemical shift range in proteins is approximately 2500 Hz. So, the appropriate gammaH2 value to cover this range is 2500/1.3 = 1923 Hz.
     

  5. Set dpwr2 to the value, which most closely matches to the calculated gammaH2.
  6. Example: dpwr2 = 40, gammaH2 = 1850 from the table in step 1.
     

  7. Calculate dmf2 by multiplying gammaH2 by a factor of 0.2472. Use the gammaH2 value chosen in step 3.
  8. Example: for dpwr2 = 40, dmf2 = 0.2472 * 1850 = 457.
     

  9. Copy the shape file 'sinx.DEC' in shapelib. Set the following parameters:

  10. dseq2 = 'sinx' dres2 = 1.0.
    Set dm2 as appropriate for the status periods of the sequence.
     
  11. Use the following procedure to calculate the decoupling profile for 'sinx' decoupling pulse with the parameters calculated using the instructions described above.
Example: