Willa
Can it be done as a company?
- hard to know
if product could be delivered
Training facility?
Glucksman
Cannot compete in scale with biotech companies
Intellectual Capital
-
- develop new
vectors
Bill Studier
With 2 - 4 people can generate lots of expression
constructs.
Have begun work in 96-well trays
Usually get rid of HexaHis - do FPLC purification
With Multiple groups feeding into Center,
have overlap / duplication problem
Willa
Core staff making proteins / helping others
to carry out expression
Larry Shapiro
Pushes for a centralized structural genomics
institute
Should focus on small genome; completely circumscribed
e.g small bacterium
Guy
outlined what our thinking is and how we can
use such a facility
Ming-Ming
maybe good to focus on small genome
Steve Elmo
Would like to broaden programs in his lab
- - tale a genomics approach to a biological focus - growth cone; salmonelle
entry -- focus on specific biological
problems.
Resource of expertise
Need to have "structural genomics" as guiding
feature for fund raising / scientific value.
Bill Studier
Put everything in T7 and take what comes.
Cuts off leader sequence. Gets ~10 - 20% of what you start with.
Larry Shapiro
Maybe make a deal with Chip Company.
Agreement with Affimetrix, Incyte for Chips
Cannot the center do both - be structural genomic center and provide resources of expertise?
Maria Tasayco
Interested in the "crumbs"; and extends the
project
Bill Studier
Center only makes sense within the context
of a structural genomics effort. Would be hard to justify a Center
if it is not part of a Structural Genomics Effort.
Chris Lima
Major focus has to be on developing new technologies.
Probably need a biochemical engineer.
Bill Studier
Goal: Least amount of effort to generate the
most information.
National lab is natural place to do it - we
hire techs rather than postdocs.
Need centralized activity.
Protein expression is centralized -- structure
solving is distributed.
