Can it be done as a company?
- hard to know if product could be delivered
Cannot compete in scale with biotech companies
- develop new vectors
With 2 - 4 people can generate lots of expression constructs.
Have begun work in 96-well trays
Usually get rid of HexaHis - do FPLC purification
With Multiple groups feeding into Center, have overlap / duplication problem
Core staff making proteins / helping others to carry out expression
Pushes for a centralized structural genomics institute
Should focus on small genome; completely circumscribed e.g small bacterium
outlined what our thinking is and how we can use such a facility
maybe good to focus on small genome
Would like to broaden programs in his lab - - tale a genomics approach to a biological focus - growth cone; salmonelle entry -- focus on specific biological
Resource of expertise
Need to have "structural genomics" as guiding feature for fund raising / scientific value.
Put everything in T7 and take what comes. Cuts off leader sequence. Gets ~10 - 20% of what you start with.
Maybe make a deal with Chip Company.
Agreement with Affimetrix, Incyte for Chips
Cannot the center do both - be structural genomic center and provide resources of expertise?
Interested in the "crumbs"; and extends the project
Center only makes sense within the context of a structural genomics effort. Would be hard to justify a Center if it is not part of a Structural Genomics Effort.
Major focus has to be on developing new technologies.
Probably need a biochemical engineer.
Goal: Least amount of effort to generate the most information.
National lab is natural place to do it - we hire techs rather than postdocs.
Need centralized activity.
Protein expression is centralized -- structure solving is distributed.