Melting Temperature Prediction Tool for PCR Primers:


Paste your primer sequence into the field below.

Paste your percent mismatching into the field below.

Paste the length of your duplex DNA in base pairs below.

The following formula will be used to calculate Tm (1).
Tm = 81.5 + [(16.6)(log{[Na+]/(1.0+0.7([Na+]))}]+[0.41(%GC)] - 500/D - P
D= length of duplex DNA   P = percent mismatching
This formula is based on Wetmur JG(1991) DNA probes: applications of the principles
of nucleic acid hybridization. Crit Rev Biochem Mol Biol 26:227-59

Promega's Tm predicictor click here