Gaetano T. Montelione Laboratory
CABM Protein NMR Laboratory

About the Lab

Gaetano T. Montelione, Principle Investigator

Gaetano T. Montelione

Jerome and Lorraine Aresty Chair in Cancer Biology
and Distinguished Professor
Center for Advanced Biotechnology and Medicine
Department of Molecular Biology and Biochemistry
Rutgers University
679 Hoes Lane
Piscataway, NJ 08854-5638

    Ph - (848) 445-9868

    Fx - (732) 235-5779

    Downloadable C.V.


Professor Gaetano Montelione holds the Jerome and Lorraine Aresty Chair in Cancer Biology Research. He is an expert in using bioinformatics, nuclear magnetic resonance (NMR), X-ray crystallography, and computational methods to determine three-dimensional protein structures. This knowledge helps scientists understand protein interactions that cause or promote diseases such as cancer and influenza, and assists in devising therapies that may be useful in combating these diseases. Dr. Montelione is co-author of more than 300 publications. Montelione is a resident member of the Center for Advanced Biotechnology and Medicine, the Cancer Institute of New Jersey and was director of the Northeast Structural Genomics Consortium of the NIH Protein Structure Initiative. Montelione is also a Visiting Professor and Foreign Expert, Jiangnan University, Wuxi, Peoples Republic of China

1980 Cornell University, Ithaca, NY, B.S. (Biochemistry)
1983 Cornell University, Ithaca, NY, M.A. (Physical Chemistry)
1985 - 1986 ETH, Zürich, Switzerland, (Molecular Biophysics)
1987 Cornell University, Ithaca, NY, Ph.D. (Physical Chemistry)
Ph.D. Advisor: Prof. H. A. Scheraga
Ph.D. co-advisor: Prof. K. Wüthrich
1987 - 1989 The University of Michigan, Ann Arbor, MI, (Molecular Biophysics)
Post-doctoral advisor: Prof. G. Wagner.


Positions and Honors

Professional Experience and Service
1988-1989 Assistant Research Professor, University of Michigan
1989-present Resident Faculty, Center for Advanced Biotechnology and Medicine
1989 Assistant Professor, Rutgers University
1992-1998 Executive Committee, Experimental NMR Conference (ENC)
1994-1998 Member, NSF Study Panel in Molecular Biophysics
1995 Associate Professor, Rutgers University
1998 Professor, Rutgers University
1999-2003 Scientific Advisory Board, NMR Facility at Madison (NMRFAM)
2000-present Director, Northeast Structural Genomics Consortium
2000-present Member, Cancer Institute of New Jersey
2000-present Associate Editor, Editorial Board Member,
PROTEINS: Structure, Function and Bioinformatics
2000-present Associate Editor, J. Struct. and Funct. Genomics
2001-present Adjunct Professor, Department of Biochemistry,
Robert Wood Johnson Medical School
2005-present Distinguished Professor, Rutgers University
2005-present NMR Advisory Task Force for Protein Data Bank
2007-present Scientific Advisory Board, World-Wide Protein Data Bank
2008-present Scientific Advisory Board, Bio Mag Res Database
2011-present Scientific Advisory Board, WeNMR European E_NMR Project
2012-present BIO Advisory Board – National Science Foundation
2012 Assessor - Critical Assessment of Protein Structure Prediction (CASP)
2012 Organizing Committee – CASP10 International Meeting
2012–present Visiting Professor and Foreign Expert, Jiangnan University,
Wuxi, Peoples Republic of China.

Honors and Award
1981-1984 National Science Foundation Graduate Research Fellowship
1981-1982 Sage Research Fellowship, Cornell University
1987 Outstanding Chemistry Graduate Student, Cornell University
1988-1989 Damon Runyon-Walter Winchell Cancer Research Fellow
1989 Searle Scholar Award
1990 Johnson & Johnson Research Discovery Award
1992-1995 American Cyanamid Award in Physical and Analytical Chemistry
1993-1998 National Science Foundation Young Investigator Award
1994-1998 Proctor and Gamble Young Investigator Award
1995-1997 Camille and Henry Dreyfus Teacher-Scholar Award
1995 Rutgers University Board of Trustees Award for Scholarly Excellence
1999 Michael & Kate Bàràny Young Investigator Awd of the Biophysical Soceity
2006 Elected Fellow of the American Association for the Advancement
of Science (AAAS)
2007 Founder, Nexomics Biosciences, Inc.
2010-present Jerome and Lorraine Aresty Chair in Cancer Biology, Rutgers University
2013 Rutgers University Football - Faculty Honoree

Selected Peer-reviewed Publications: Dr. Montelione is co-author of over 350 publications. For the complete list see our Publications page  

1. Baran, M.C.; Huang, Y.J.; Moseley, H.N.; Montelione, G.T. Chemical Reviews 2004, 104:3451-3555. Automated analysis of protein NMR assignments and structures.

2. Yin, C.; Khan, J.A.; Swapna, G.V.T.; Ertekin, A.; Krug, R.M.; Tong, L.; Montelione, G.T. J. Biol. Chem. 2007, 282:20584-20592. Conserved surface features form the double-stranded RNA-binding site of non-structural protein 1 (NS1) from Influenza A and B viruses.

3. Huang, Y.J.; Hang, D.; Lu, L.J.; Tong, L.; Gerstein, M.B.; Montelione, G.T. Mol. Cell. Proteomics 2008, 7:2048-2060. Targeting the human cancer pathway protein interaction network by structural genomics.

4. Das, K.; Ma, L-C.; Xiao, R.; Radvansky, B.; Aramini, J.; Zhao, L.; Marklund, J.; Kuo, R-L.; Twu, K.Y.; Arnold, E.; Krug, R.M.; Montelione, G.T. Proc. Natl. Acad. Sci. U.S.A. 2008, 105:13092-13097. Structural basis for suppression by influenza A virus of a host antiviral response.

5. Aramini, J.M.; Tubbs, J.L.; Kanugula, S.; Rossi, P.; Ertekin, A.; Maglaqui, M.; Hamilton, K.; Ciccosanti, C.T.; Jiang, M.; Xiao, R.; Soong, T.-T.; Rost, B.; Acton, T.B.; Everett, J.K.; Pegg, A.E.; Tainer, J.A.; Montelione, G.T. J. Biol. Chem. 2010, 285:13736-13741. Structural basis of O6-alkylguanine recognition by a bacterial alkyltransferase-like DNA repair protein.

6. Raman, S.; Lange, O.F.; Rossi, P.; Tyka, M.; Wang, X.; Aramini, J.; Liu, G.; Ramelot, T.; Eletsky, A.; Szyperski, T.; Kennedy, M.; Prestegard, J.; Montelione, G.T.; Baker, D. Science 2010, 327:1014-1018. NMR structure determination for larger proteins using backbone-only data.

7. Montelione, G.T.; Szyperski, T.; Curr. Opin. Drug Discovery 2010, 13: 335 - 349. Advances in protein NMR impacting drug discovery provided by the NIGMS Protein Structure Initiative.

8. Guan, R.; Ma, L-C; Leonard, P.G.; Amer, B.R.; Sridharan, H.; Zhao, C.; Krug, R.M.; Montelione, G.T. Proc. Natl. Acad. Sci. U.S.A. 2011, 108:13468–13473. Structural basis for the sequence-specific recognition of human ISG15 by the NS1 protein of influenza B virus.

9. Mao, B.; Guan, R.; Montelione, G.T. Structure 2011, 19:757-766. Improved technologies now routinely provide protein NMR structures useful for molecular replacement.

10. Ertekin, A.; Aramini, J.M.; Rossi, P.; Leonard, P.G.; Janjua, H.; Xiao, R.; Maglaqui, M.; Lee, H.-W.; Prestegard, J.H.; Montelione, G.T. J. Biol. Chem. 2012, 287:16541-16549. Human cyclin dependent kinase 2 associated protein 1 is dimeric in its disulfide-reduced state, with natively disordered N-terminal region.

11. Koga, N.; Tatsumi-Koga, R.; Liu, G.; Xiao, R.; Acton, T.B.; Montelione, G.T.; Baker, D. Nature 2012, 491:222-227. Principles for designing ideal protein structures.

12. Aramini, J.M.; Hamilton, K.; Rossi, P.; Ertekin, A.; Lee, H.-W.; Lemak, A.; Wang, H.; Xiao, R.; Acton, T.B.; Everett, J.K.; Montelione, G.T. Biochemistry 2012, 51:3705-3707. Solution NMR structure, backbone dynamics, and heme-binding properties of a novel cytochrome c maturation protein CcmE from Desulfovibrio vulgaris.

13. Lange, O.F.; Rossi, P.; Sgourakis, N.; Song, Y.; Lee, H.-W.; Aramini, J.M.; Ertekin, A.; Xiao, R.; Acton, T.B.; Montelione, G.T.; Baker, D. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:10873-10878. Determination of solution structures of proteins up to 40 KDa using CS-Rosetta with sparse NMR data from deuterated samples.

14. Huang, Y.; Rosato, A.; Singh, G.; Montelione, G.T. Nucleic Acids Research 2012, 40:W542-546. RPF – A quality assessment tool for protein NMR structures

15. Rosato, A.; Tejero, R.; Montelione, G.T. Current Opinions in Structural Biology 2013, 23:715-724. Quality assessment of protein NMR structures.

16. Montelione, G.T.; Nilges, M.; Bax, A.; Güntert, P.; Herrmann, T.; Richardso J.S.; Schwieters, C.; Vranken, W.F.; Vuister, G.W.; Wishart, D.S.; Berman, H. Kleywegt, G.J.; Markley, J.L. Structure 2013, 21:1563-1570. Recommendations of the wwPDB NMR Validation Task Force.

17. Huang, Y.P.; Mao, B.; Aramini, J.; Montelione, G.T. PROTEINS: Struc. Funct. Genomics 2014, 82: Suppl 2:43-56. Assessment of template based protein structure predictions in CASP10.

18. Mao, B.; Tejero, R.; Baker, D.; Montelione, G.T. J. Amer. Chem. Soc. 2014, 136:1893-1906. Protein NMR structures refined with Rosetta have higher accuracy relative to corresponding X-ray crystal structures.

19. Aramini, J.M.; Hamilton, K.; Ma, L-C.; Swapna, G.V.T.; Leonard, P.G.; Ladbury, J.E.; Krug, R.M.; Montelione, G.T. Structure 2014, 22:515-525. 19F NMR reveals multiple conformations at the dimer interface of the NS1 effector domain from influenza A virus.

20. Aiyer, S.; Swapna, G.V.T.; Malani, N.; Aramini, J.M.; Schneider, W.M.; Plumb, M.R.; Ghanem, M.; Larue, R.C.; Sharma, A.; Studamire, B.; Kvaratskhelia, M.; Bushman, F.D.; Montelione, G.T.; Roth, M.J. Nucleic Acids Research 2014, 42:5917-5928. Altering murine leukemia virus integration through disruption of the integrase and BET protein family interaction

21. Huang, Y.P.; Mao, B.; Xu, F.; Montelione, G.T. J. Biomol. NMR 2015, 62:439-451. Guiding automated NMR structure determination using a global optimization metric, the NMR DP score.

22. Everett, J.K.; Tejero, R.; Murthy, S.B.K.; Acton, T.B.; Aramini, J.M.; Baran, M.C.; Benach, J.; Cort, J.R.; Eletsky, A.; Forouhar, F.; Guan, R.; Kuzin, A.P.; Lee, H.-W.; Liu, G.; Mani, R.; Mao, B.; Mills, J.L.; Montelione, A.F.; Pederson, K.; Powers, R.; Ramelot, T.; Rossi, P.; Seetharaman, J.; Snyder, D.; Swapna, G.V.T.; Vorobiev, S.M.; Wu, Y.; Xiao, R.; Yang, Y.; Arrowsmith, C.H.; Hunt, J.F.; Kennedy, M.A.; Prestegard, J.H.; Szyperski, T.; Tong, L.; Montelione, G.T. Protein Science 2015, 25:30-45. A community resource of experimental data for NMR – X-ray crystal structure pairs.

23. Tang, Y.; Huang, Y.P.; Hopf, T.A.; Sanders, C.; Marks, D.S.; Montelione, G.T. Nature Methods 2015, 12:751-754 Protein structure determination by combining sparse NMR spectroscopy data with evolutionary couplings.

24. Ma, L-C.; Guan, R.; Hamilton, K.; Aramini, J.; Mao, L.; Wang, S.; Krug, R.M.; Montelione, G.T. Structure 2016, 24:1562-1572. A second RNA-binding site in the NS1 protein of influenza B virus.

25. Boël, G.; Letso, R.; Neely, H.; Price, W.N.; Wong, K.H.; Su, M.; Luff, J.; Valecha, M.; Everett, J.; Acton, T.B.; Xiao, R.; Montelione, G.T.; Aalberts, D.P.; Hunt, J.F. Nature 2016, 529:358-363. Codon influence on protein expression in E. coli correlates to mRNA levels.

26. Marcos, E.; Basanta, B.; Chidyausiku, T.M.; Tang, Y.; Oberdorfer, G.; Liu, G.; Swapna, G.V.T.; Guan, R.; Silva, D.-A.; Dou, J.; Pereira, J.H.; Xiao, R.; Banumathi Sankaran, B.; Zwart, P.H.; Montelione, G.T.; Baker, D. Science 2017, 355:201-206. Principles for designing proteins with cavities formed by curved β-sheets.

27. Gibbs, A.C.; Steele, R.; Liu, G, Tounge, B.A.; Montelione. G. Biochemistry 2018, 57:1591-1602. Inhibitor bound dengue NS2B-NS3pro reveals multiple dynamic binding modes.

Update: 06/26/18